electrical resistance meter  (World Precision Instruments)

Journal: Communications Biology

Article Title: Cx43 levels guide apicobasal polarity in regenerating airway epithelial cells

doi: 10.1038/s42003-026-10413-7

Figure Lengend Snippet: a Representative top view of 3D reconstructed confocal images showing giantin immunostaining (red), performed in polarized CTL and CFTR KD Calu-3 cells depleted or not for Cx43. Scale bar: 20 μm. Quantification of Golgi dispersal ( b 4 biological replicates, **** p < 0.0001) and the total area covered by the fluorescent signal ( c 4 biological replicates, ** p = 0.0065, *** p = 0.0003). d Representative top view of 3D reconstructed confocal images showing ZO-1 immunostaining (green), performed in polarized CTL and CFTR KD depleted or not for Cx43. Scale bar: 40 μm. e Quantification of the ZO-1 immunofluorescence (3 biological replicates, n = 8, * p = 0.0206, ** p = 0.0094). n represents the total number of analyzed images per condition. f Transepithelial electrical resistance (TEER) was measured to assess the integrity and functional status of tight junctions in 3 biological replicates of polarized CTL and CFTR KD cells depleted or not for Cx43. TEER values are expressed as Ω.cm² (4 biological replicates, n = 21, **** p < 0.0001). n represents the total number of Transwell® inserts analyzed per condition. g Representative Western blot depicting fibronectin (FN1) detection in the apical secretome collected 24 h after incubation of saline on top of polarized CTL and CFTR KD cells, depleted or not for Cx43. Schematic in the left panel illustrates how the secretomes were collected. h Fibronectin band intensity was quantified for all collected secretomes ( n = 4 biological replicates, **** p < 0.0001). i Representative confocal images of fibronectin (cyan) immunostaining in polarized CTL and CFTR KD Calu-3 cells depleted or not for Cx43. Nuclei were stained with DAPI (blue). For each condition, the left panels show the 3D projection of z-stack confocal images providing a comprehensive view of the apical deposition of fibronectin, and the images on the right depict a single focal plane image at the basal side of the epithelium. Scale bar: 50 μm. Data are presented as mean ± s.e.m.; two-way ANOVA with post hoc Tukey’s multiple comparison test ( b , c , e , f , h ).

Article Snippet: TEER values were recorded from primary HAECs or Calu-3 cells cultured on Transwell inserts at ALI using an electrical resistance meter (EVOM; World Precision Instruments).

Techniques: Confocal, Immunostaining, Immunofluorescence, Functional Assay, Western Blot, Incubation, Saline, Staining, Comparison

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Host-pathogen-immune interactions in an air-liquid interface airway model

doi: 10.3389/fcimb.2026.1788554

Figure Lengend Snippet: Effect of Pseudomonas aeruginosa infection in mono- and dual-cell culture models. (A) IL-8 quantification (pg/mL) after 16 h of infection with (P) aeruginosa. (B) Fold change of Transepithelial Electrical Resistance (TEER) (Ω·cm 2 ) in both models after infection with (P) aeruginosa for 16 h. (C) Bacterial load in colony-forming units (CFU/mL) across the different compartments of the dual-cell air-liquid-interface model, apical, attached (bacteria present within the epithelial cells or firmly attached to the surface), and the basolateral compartment. (D) Orthogonal and cross-sectional views of the mono- and dual-cell culture model after 16 h of infection with (P) aeruginosa (scale bar: 100 µm). Left image, a mono-cell culture infection model showing ciliated cells (red, acetylated alpha-tubulin), (P) aeruginosa (green, sfGFP), and cell nuclei (blue, DAPI); right image shows a dual-cell culture model with ciliated cells (red, acetylated alpha-tubulin), (P) aeruginosa (green, sfGFP), cell nuclei (blue, DAPI), and macrophages (yellow, CD14). All Images were taken at 40X magnification. Data represent mean ± SD from three biological replicates, each with three technical replicates. Statistical significance was determined by one-way ANOVA with Tukey ’ s multiple comparisons test for IL-8, TEER and CFU comparisons, and is indicated as *p ≤ 0.05, **p ≤ 0.001, *** p ≤ 0.0001, and ****p < 0.0001.

Article Snippet: In addition, Transepithelial electrical resistance (TEER) was measured using a voltohmmeter (e.g., EVOM2, World Precision Instruments) with STX electrodes according to the manufacturer’s instructions.

Techniques: Infection, Cell Culture, Bacteria